Laboratory of Genomics and Transcriptomics
Organoids show great potential in many applications, including drug discovery, toxicology, and disease modeling. Primary tumors can be cultured ex vivo as organoids, containing the malignant tumor cells and the supporting cells from the tumor environment. Primary 3-D organoid culture of tumors is an attractive platform for studies of solid tumors. 3-D organoids from patients’ tumors recapitulate in vivo tissue structural organization, functional differentiation, chemical and mechanical signals and can be used for ex vivo drug screening prior to treatment. Glioblastoma is a devastating disease that despite all the molecular information gathered so far, its optimal management remains elusive due to the absence of validated target from clinical studies.
Serena Barachini is involved in a project aimed to perform optical metabolic imaging of 3D organoids derived from primary human glioblastoma tumors to assess therapeutic response and disease progression. Optical metabolic imaging shows potential as a high-throughput screen to test the efficacy of a panel of drugs to select optimal drug combinations. Glioblastoma organoids responsive and resistant to the therapy will be molecularly characterized, with whole genome and whole trascriptome analysis, to identify factors involved in tumor progression and therapy responsiveness.
Serena Barachini received her Master’s degree in Biological Science at the University of Pisa, Italy, and her PhD in Experimental Medicine at the University of Rome, where she carried out part of her research at the Policlinico Umberto I in the Cell Therapy Unit and Laboratory of Tumor Immunology. In 2007, she obtained the Specialization in Clinical Pathology from Medical School of the University of Pisa. After the Specialization, she worked at Therapeutic Cell Manipulation Laboratory, Hematology Division, S. Chiara University Hospital, where her research focused on isolation and morpho-functional characterization of mesodermal progenitor’s cells from bone marrow-derived, cord blood, adipose tissue and dental pulp.